Keyhole Limpet Hemocyanin （KLH） is one of the most commonly used carriers in the conjugation of peptides for antibody production。 Mariculture keyhole limpet hemocyanin （mcKLH） is a hemocyanin from the Concholepas concholepas mollusk with immunogenic properties similar to KLH but is more stable and efficient as a carrier protein for the production of antibodies to haptens and peptides。 It contains numerous sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers。 mcKLH is currently the industry standard for antibody production against a hapten or peptide。
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描述
This ReadiLink KLH Conjugation kit is primarily optimized for the simple preparation of hapten-carrier conjugates for immunization and antibody production。 The ReadiLink KLH Conjugation kit is one-step conjugation of a hapten to a carrier protein using the carboxyl-reactive carbodiimide as the crosslinker。 The resulting conjugate is used for eliciting an immune response and antibody production against the hapten。 The carboxyl-reactive carbodiimide reacts with exposed carboxyl and amino groups on peptides and proteins to form stable bonds。 These kits contain mcKLH formulated in buffers compatible with the carboxyl-reactive carbodiimide reactions and desalt spin columns， which offer exceptional protein recovery by simple centrifugation step。
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