Rac1 Pulldown 活化检测试剂盒现货-Rac1 Pulldown Activation Assay Kit-细胞分析试剂盒-欧宝体育官方科技有限公司

PRODUCT CENTER 细胞分析试剂盒

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中文名称: Rac1 Pulldown 活化检测试剂盒现货

英文名字: Rac1 Pulldown Activation Assay Kit

供应商: Cytoskeleton

欧宝体育官方货号: BK035

欧宝体育官方报价: ￥询价/KIT50assays欧宝体育官方:

欧宝体育官方说明书

购买方式: Cytoskeleton中国区现货中心已经建立，大部分欧宝体育官方中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与欧宝体育官方电话联系400-6800-868

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背景资料: 该Rac1 Pulldown 活化检测试剂盒欧宝体育官方是Cytoskeleton品牌原装进口，Cytoskeleton品牌是细胞骨架研究专家，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。欧宝体育官方作为中国区域的总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立专业技术支持团队的高科技生物企业。可以为您提供及时的咨询响应，专业的欧宝体育官方和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。欧宝体育官方致力于为您提供有价值的欧宝体育官方和服务，在意您的成功！

欧宝体育官方描述: 收到该Rac1 Pulldown 活化检测试剂盒Rac1 Pulldown Activation Assay Kit欧宝体育官方后，查看厂家提供的欧宝体育官方说明书详细介绍。

欧宝体育官方特点: The Rho switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of the GTPases is of obvious biological significance and is a subject of intense investigation. The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Rac and Cdc42 protein activation. The assay uses the Cdc42/Rac Interactive Binding (CRIB) region (also called the p21 Binding Domain, PBD) of the Cdc42 / Rac effector protein, p21 activated kinase 1 (PAK). The CRIB/PBD protein motif has been shown to bind specifically to the GTP-bound form of Rac and/or Cdc42 proteins. The fact that the PBD region of PAK has a high affinity for both GTP-Rac and GTP-Cdc42 and that PAK binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of both Rac and Cdc42 make it an ideal tool for affinity purification of GTP-Rac and GTP-Cdc42 from cell lysates. The PAK-PBD protein supplied in this kit corresponds to residues 67-150. This includes the highly conserved CRIB region (aa 74-88) plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42. The PAK-PBD is in the form of a GST fusion protein, which allows one to "pull-down" the PAK-PBD/GTP-Rac (or GTP-Cdc42) complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Rac or Cdc42 activation in cells. The amount of activated Rac is determined by a Western blot using a Rac-specific antibody.

保存建议: 厂家推荐常温运输，收到欧宝体育官方后建议保存于4°C。

其他: Cytoskeleton公司成立于1993年，专注于生物化学和细胞过程研究中的纯化蛋白和便捷试剂盒开发与生产。公司提供药物筛选、信号转导、蛋白质转录后修饰（PTM）、细胞骨架研究相关的系列试剂盒和欧宝体育官方，尤其以细胞骨架相关研究见长，既能满足于样品较少的科学研究，也可以用于小规模筛选研究和高通量大规模筛选研究。此外，公司还提供微管蛋白，肌动蛋白，小G蛋白，GAPs，GEFs等现有欧宝体育官方的药物筛选服务。