【干冰】Small Sample Targeted ChIP Kit - KOA0886-【干冰】Small Sample Targeted ChIP Kit - KOA0886-免疫学试剂盒-欧宝体育官方科技有限公司

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中文名称: 【干冰】Small Sample Targeted ChIP Kit - KOA0886

英文名字: 【干冰】Small Sample Targeted ChIP Kit - KOA0886

供应商: Rockland

欧宝体育官方货号: KOA0886

欧宝体育官方报价: ￥询价/1Kit欧宝体育官方:

欧宝体育官方说明书

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背景资料: Association between proteins and DNA is a major mechanism in many vital cellular functions such as gene transcription and epigenetic silencing. It is crucial to understand these interactions and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is a technique to analyse the association of proteins with specific genomic regions in intact cells. ChIP can be used to study changes in epigenetic signatures, chromatin remodelling and transcription regulator recruitment to specific genomic sites. In ChIP, living cells are first fixed with a reversible crosslinking agent to stabilize protein-DNA interactions. The most widely used reagent to fix cells is formaldehyde which generates covalent bonds between amino or imino groups of proteins and nucleic acids. Formaldehyde treatment crosslinks both DNA-protein as well as protein-protein complexes. However conventional ChIP protocols require high numbers of cells (hundreds of thousands cells at least) limiting the application for ChIP technology to few cell samples. More recently, ChIP assays on smallest amount of cells have been reported. Nevertheless the procedure requires tedious optimization of several reaction conditions to face the increased background observed in ChIP performed with reduced amount of cells. That might consequently lead to considerable time and lab expenditures. To reduce these tedious steps, Small Sample Targeted ChIP Kit has optimized reagents and protocol to enable successful ChIP on as few as 10 000 cells. Moreover, the Small Sample Targeted ChIP Kit protocol has been thoroughly optimised for ChIP followed by high-throughput sequencing on Illumina® Next-Gen sequencers.

欧宝体育官方描述: The different steps of the ChIP assay are cell fixation (crosslinking), chromatin shearing, immunoprecipitation, reverse crosslinking followed by DNA purification and analysis of the immunoprecipitated DNA. Suitable for 10 reactions.

欧宝体育官方特点: 该【干冰】Small Sample Targeted ChIP Kit - KOA0886的详细信息查看Rockland提供的欧宝体育官方说明书。

保存建议: See kit insert for components stored at 2-8°C and -20°C. Do not freeze Protein A and magnetic beads.

其他: Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。