The peroxidase-antiperoxidase (PAP) approach utilizes a complex of enzyme and antibody to facilitate amplification of signal, indirectly detecting the amount of primary antibody used in an assay. Generated in Goat, the PAP complex can be utilized in any assay that utilizes a goat primary antibody. With the addition of a secondary antibody that recognizes goat, the reporter PAP complex allows for amplification of the luminescent signal (as three molecules of enzyme are bound to every two immunoglobulins).
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描述
Suitable for use in peroxidase enzymatic labeling experiments. After reconstitution, dilute sample with PBS containing 5% normal serum from same host bridging antibody.
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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